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Goat Anti-HOXA9 (aa49-60) Antibody (EB10045)

Related Products: EBP10045 - Immunizing Peptide, EBP10046 - Immunizing Peptide, Goat Anti-HOXA9 (aa37-50) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10045 Goat Anti-HOXA9 (aa49-60) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10045 1
EB10045 (2µg/ml) staining of Moue Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ABD-B, homeo box A9, homeobox A9, homeobox protein HOXA9, homeodomain protein HOXA9, HOX1, HOX1.7, HOX1G, MGC1934, HOXA9
Official Symbol: HOXA9
Accession Number(s): NP_689952.1
Human GeneID(s): 3205

Immunogen

Peptide with sequence PDFSPCSFQSKA, from the internal region of the protein sequence according to NP_689952.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 36kDa band observed in Mouse Spleen lysates (calculated MW of 29.9kDa according to NP_034586.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Quéré et al, Blood. 2011 Jun 2;117(22):5918-30. PMID: 21471525). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat. Dog. Cow

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