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Goat Anti-HSPC150 / UBE2T Antibody (EB05765)

Related Products: EBP05765 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05765 Goat Anti-HSPC150 / UBE2T Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05765 1

EB05765 optimised QC. Primary incubation 1 hour at room temperature.

Image A: K562 cell lysate at primary Ab concentration 0.1µg/ml, Image B: THP-1 cell lysate at primary Ab concentration 0.3µg/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB05765 2

EB05765 optimised QC. Primary incubation 1 hour at room temperature.

Images A, B, C, D: A431, HEK293, HeLa, Jurkat nuclear cell lysate at primary Ab concentration 0.3µg/ml, Image E: HepG2 nuclear cell lysate at primary Ab concentration 0.1µg/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB05765 3
EB05765 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05765 4
EB05765 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05765 5
EB05765 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBE2T, HSPC150, PIG50, HSPC150 protein similar to ubiquitin-conjugating enzyme, ubiquitin conjugating enzyme, ubiquitin-conjugating enzyme E2T (putative), ubiquitin-conjugating enzyme E2T
Official Symbol: UBE2T
Accession Number(s): NP_054895.1; NP_001297255.1
Human GeneID(s): 29089

Immunogen

Peptide with sequence C-QLVGIEKKFHPDV, from the C Terminus of the protein sequence according to NP_054895.1; NP_001297255.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 23-24kDa band observed in nuclear lysates of cell lines HeLa, Jurkat, HEK293, HepG2 and in THP-1 cell lysates, and approx. 25kDa in lnuclear lysates of cell line A431 and in K562, cell lysatess (calculated MW of 22.5kDa according to NP_054895.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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