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Goat Anti-ICBP90 / UHRF1 Antibody (EB05943)

Related Products: EBP05943 - Immunizing Peptide, EBP09807 - Immunizing Peptide, Goat Anti-UHRF1 / ICBP90 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05943 Goat Anti-ICBP90 / UHRF1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05943 1
EB05943 (2µg/ml) staining of nuclear Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: UHRF1, ubiquitin-like, containing PHD and RING finger domains, 1, Np95, ICBP90, FLJ21925, transcription factor ICBP90, RNF106, MGC138707, RING finger protein 106, inverted CCAAT box-binding protein of 90 kDa, nuclear zinc finger protein Np95, transcription factor ICBP90, ubiquitin-like with PHD and ring finger domains 1, hNP95, E3 ubiquitin-protein ligase UHRF1
Official Symbol: UHRF1
Accession Number(s): NP_001041666.1; NP_037414.3
Human GeneID(s): 29128
Non-Human GeneID(s): 18140 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms.

Immunogen

Peptide with sequence C-LNQLFPGYGNGR, from the C Terminus of the protein sequence according to NP_001041666.1; NP_037414.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 100kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 91.1kDa according to NP_037414.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Hopfner, Cancer Res. 2000 Jan 1;60(1):121-8. PMID: 10646863). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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