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Goat Anti-IFNAR2 Antibody (EB08349)

Related Products: EBP08349 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08349 Goat Anti-IFNAR2 Antibody Pep-ELISA, FC, IHC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08349 1
EB08349 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
EB08349 2
EB08349 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08349 3
EB08349 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: IFNAR2, interferon (alpha, beta and omega) receptor 2, IFN-R, IFN-alpha-REC, IFNABR, IFNARB
Official Symbol: IFNAR2
Accession Number(s): NP_000865.2 ; NP_997467.1; NP_997468.1
Human GeneID(s): 3455
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_000865.2 ; NP_997467.1; NP_997468.1 )

Immunogen

Peptide with sequence SYDSPDYTDESCT, from the internal region of the protein sequence according to NP_000865.2 ; NP_997467.1; NP_997468.1 .

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 28kDa band in Human Uterus lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 37.4kDa according to NP_000865.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Prostate and Placenta. Recommended concentration: 5µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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