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Goat Anti-ING2 / ING1L Antibody (EB05452)

Code Name Applications Availability Size Tested species Price Grade  
EB05452 Goat Anti-ING2 / ING1L Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB05452 1
EB05452 staining (2µg/ml) of HepG2 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ING2, ING1L, p33ING2, inhibitor of growth family, member 1-like, inhibitor of growth 1-like, inhibitor of growth family, member 2
Official Symbol: ING2
Accession Number(s): NP_001555.1
Human GeneID(s): 3622

Immunogen

Peptide with sequence C-DKSTEKTKKDRRSR, from the C Terminus of the protein sequence according to NP_001555.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 30kDa band observed in hepatocarcinoma HEPG2 lysates (calculated MW of 32.8kDa according to NP_001555). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow