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Goat Anti-ITGAM / CD11B Antibody (EB07825)

Related Products: EBP07825 - Immunizing Peptide, Goat Anti-ITGAM / CD11B Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB07825 Goat Anti-ITGAM / CD11B Antibody Pep-ELISA, IF, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07825 1
EB07825 Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07825 2
EB07825 Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07825 3
EB07825 (8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07825 4
EB07825 Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ITGAM, integrin, alpha M (complement component 3 receptor 3 subunit), CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, Integrin, alpha-M (complement component receptor-3, alpha), antigen CD11B (p170), macrophage antigen, alpha polypeptide, antigen CD11b (p170), complement component receptor 3, alpha, integrin alpha M, integrin, alpha M (complement component receptor 3, alpha), CD11b (p170), macrophage antigen alpha polypeptide, macrophage antigen alpha polypeptide, neutrophil adherence receptor alpha-M subunit
Official Symbol: ITGAM
Accession Number(s): NP_000623.2
Human GeneID(s): 3684
Non-Human GeneID(s): 16409 (mouse), 25021 (rat)
Important Comments: The immunizing peptide represents part of the extra-cellular domain.

Immunogen

Peptide with sequence C-RNDGEDSYRTQ, from the internal region of the protein sequence according to NP_000623.2 .

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 6-8µg/ml.

Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of U937 and THP-1 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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