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Goat Anti-KLF4 / GKLF Antibody (EB05473)

Related Products: EBP05473 - Immunizing Peptide, Goat Anti-KLF4 / GKLF Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB05473 Goat Anti-KLF4 / GKLF Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05473 1
EB05473 (0.1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: KLF4, GKLF, Kruppel-like factor 4 (gut), EZF, endothelial Kruppel-like zinc finger protein
Official Symbol: KLF4
Accession Number(s): NP_004226.2
Human GeneID(s): 9314

Immunogen

Peptide with sequence AVSDALLPSFST-C, from the N Terminus of the protein sequence according to NP_004226.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Experiments gave strong bands at 75-90kDa and a faint 50-kDa band in Human Duodenal lysate after 0.1µg/ml antibody staining. These bands correspond to earlier findings in literature (Piccinni et al, J. Biol. Chem., Vol. 279 (10), 9103-9114). All these bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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