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Goat Anti-KLK2 Antibody (EB07673)

Related Products: EBP07673 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07673 Goat Anti-KLK2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07673 1
EB07673 (1µg/ml) staining of HeLa cell lysate (A) + peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07673 2
EB07673 (8µg/ml) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07673 3
EB07673 Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
EB07673 4
EB07673 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: KLK2, kallikrein-related peptidase 2, KLK2A2, MGC12201, hK2, glandular kallikrein 2, kallikrein 2, prostatic
Official Symbol: KLK2
Accession Number(s): NP_005542.1; NP_001002231.1
Human GeneID(s): 3817
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005542.1; NP_001002231.1).

Immunogen

Peptide with sequence C-KHQSLRPDEDSSHD, from the internal region of the protein sequence according to NP_005542.1; NP_001002231.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 26kDa band observed in lysates of cell line HeLa, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 28.7 according to NP_005542.1 and 24.7kDa according to NP_001002231.1 ). Recommended concentration:0.5-2 µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 2.5-8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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