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Goat Anti-Lactadherin Antibody (EB12248)

Code Name Applications Availability Size Tested species Price Grade  
EB12248 Goat Anti-Lactadherin Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB12248 1
EB12248 (1µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MFGE8, milk fat globule-EGF factor 8 protein, BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47, O-acetyl disialoganglioside synthase, breast epithelial antigen BA46, lactadherin, medin, sperm associated antigen 10, sperm surface protein hP47
Official Symbol: MFGE8
Accession Number(s): NP_005919.2; NP_001108086.1
Human GeneID(s): 4240
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005919.2; NP_001108086.1).

Immunogen

Peptide with sequence C-KNNSIPDKQITAS, from the internal region of the protein sequence according to NP_005919.2; NP_001108086.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40kDa band observed in lysates of cell line MCF7 (calculated MW of 43.1kDa according to NP_005919.2, and 37.5 according to NP_001108086.1 ). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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