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Goat Anti-LGP2 Antibody (EB07981)

Related Products: EBP07981 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07981 Goat Anti-LGP2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07981 1
EB07981 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07981 2
EB07981 (8µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07981 3
EB07981 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: DHX58, LGP2, DEXH (Asp-Glu-X-His) box polypeptide 58, D11LGP2, D11lgp2e, LGP2, RNA helicase LGP2, ortholog of mouse D11lgp2
Official Symbol: DHX58
Accession Number(s): NP_077024.2
Human GeneID(s): 79132

Immunogen

Peptide with sequence DFLQHCAENLSD, from the C Terminus of the protein sequence according to NP_077024.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 80kDa band observed in Human Liver lysates (calculated MW of 76.6kDa according to NP_077024.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 24kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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