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Goat Anti-LNK / SH2B3 Antibody (EB06454)

Related Products: EBP05122 - Immunizing Peptide, EBP06454 - Immunizing Peptide, Goat Anti-LNK Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06454 Goat Anti-LNK / SH2B3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06454 1
EB06454 (0.3µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06454 2
HEK293 overexpressing SH2B3 (RC218359) and probed with EB06454 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: SH2B3, LNK, SH2B adaptor protein 3, lymphocyte adaptor protein
Official Symbol: SH2B3
Accession Number(s): NP_005466.1
Human GeneID(s): 10019

Immunogen

Peptide with sequence NGPALQPSSPC, from the N Terminus of the protein sequence according to NP_005466.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 70kDa band observed in lysates of cell line MOLT4 (calculated MW of 63.2kDa according to NP_005466.1). In transfected HEK293 transiently expressing SH2B3 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1.0 µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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