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Goat Anti-MDA5 / IFIH1 Antibody (EB05965)

Related Products: EBP05965 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05965 Goat Anti-MDA5 / IFIH1 Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human, Monkey $450.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05965 1
EB05965 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
EB05965 2
EB05965 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: IFIH1, MDA5, MDA-5, Hlcd, MGC133047, IDDM19, melanoma differentiation associated protein-5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, interferon induced with helicase C domain 1, helicard
Official Symbol: IFIH1
Accession Number(s): NP_071451.2
Human GeneID(s): 64135

Immunogen

Peptide with sequence SNGYSTDENFRYL-C, from the N Terminus of the protein sequence according to NP_071451.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: This antibody has been successfully used in Western blot on African Green Monkey: Sato S et al, Arthritis Rheum. 2009 Jul;60(7):2193-200. PMID: 19565506.
IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Monkey
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in Western blot on African Green Monkey:
Sato S, Hoshino K, Satoh T, Fujita T, Kawakami Y, Fujita T, Kuwana M.
RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease.
Arthritis Rheum. 2009 Jul;60(7):2193-200.
PMID: 19565506

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