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Goat Anti-Mindbomb 1 / MIB1 Antibody (EB11532)

Related Products: EBP11532 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11532 Goat Anti-Mindbomb 1 / MIB1 Antibody Pep-ELISA, WB-Trf, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11532 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MIB1 with C-terminal MYC tag probed with EB11532 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB11532 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
EB11532 2
EB11532 (2µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DAPK-interacting protein 1, DIP-1, DKFZp686I0769, DKFZp761M1710, E3 ubiquitin-protein ligase MIB1, FLJ90676, MGC129659, MGC129660, MIB, MIB1, mind bomb homolog 1, mindbomb homolog 1 (Drosophila), OTTHUMP00000162549, ubiquitin ligase mind bomb, ubiquitin ligase protein MIB1, zinc finger ZZ type with ankyrin repeat domain protein 2, ZZANK2, ZZZ6
Official Symbol: MIB1
Accession Number(s): NP_065825.1
Human GeneID(s): 57534
Non-Human GeneID(s): 225164 (mouse), 307594 (rat)

Immunogen

Peptide with sequence C-DKDNTNVNADVQKLQ, from the internal region (near C Terminus) of the protein sequence according to NP_065825.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing Human MIB1 (myc and DYKDDDDK tagged), a band of approx. 135kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1µg/ml.
IHC: In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in the neuronal bodies, on one side of the nucleus. Recommended concentration, 2-4µg/ml.

Purchase the +ve control! The transfected lysate used in QC is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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