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Goat Anti-MRP8 / ABCC11 Antibody (EB08705)

Related Products: EBP08705 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08705 Goat Anti-MRP8 / ABCC11 Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08705 1
EB08705 (0.5µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08705 2
EB08705 (3.75µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08705 3
EB08705 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08705 4
EB08705 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ABCC11, ATP-binding cassette transporter MRP8, ATP-binding cassette, sub-family C (CFTR/MRP), member 11, EWWD, MRP8, WW, ATP-binding cassette protein C11, ATP-binding cassette, sub-family C, member 11, multi-resistance protein 8
Official Symbol: ABCC11
Accession Number(s): NP_115972.2; NP_660187.1; NP_001357425.1
Human GeneID(s): 85320

Immunogen

Peptide with sequence C-KVVEFDRPEVLRK, from the internal region (near C Terminus) of the protein sequence according to NP_115972.2; NP_660187.1; NP_001357425.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 125kDa band observed in Human Testis lysates and in preliminary testing of Breast lysate (calculated MW of 150kDa according to NP_660187.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-1.5µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Breast. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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