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Code Name Applications Availability Size Tested species Price Grade  
EB08335 Goat Anti-MSH5 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB08335 1
EB08335 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08335 2
EB08335 (2.5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MSH5, mutS homolog 5 (E. coli), XXbac-BCX40G17.5, DKFZp434C1615, G7N: MGC2939, MutSH5, NG23, mutS homolog 5
Official Symbol: MSH5
Accession Number(s): NP_079535.3 ; NP_751897.1; NP_002432.1
Human GeneID(s): 4439
Non-Human GeneID(s): 17687 (mouse), 294252 (rat)
Important Comments: This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.

Immunogen

Peptide with sequence GDLHCEIRDQET, from the internal region of the protein sequence according to NP_079535.3 ; NP_751897.1; NP_002432.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 38kDa band in Human Testis, Tonsil and Thymus lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kDa according to the smallest reported isoform NP_002432.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Testis and Thymus. Recommended concentration: 2.5-3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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