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Goat Anti-MUNC18 / STXBP1 (isoform a) Antibody (EB06140)

Related Products: EBP06140 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06140 Goat Anti-MUNC18 / STXBP1 (isoform a) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06140 1
EB06140 (0.3µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06140 2
EB06140 (5µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06140 3
EB06140 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: STXBP1, MUNC18-1, UNC18, hUNC18, syntaxin binding protein 1, Hunc18, rbSec1, syntaxin-binding protein 1, RP11-56D16.3, EIEE4, FLJ37475, RBSEC1
Official Symbol: STXBP1
Accession Number(s): NP_003156.1
Human GeneID(s): 6812
Non-Human GeneID(s): 20910 (mouse)
Important Comments: This antibody is expected to recognize isoform a (NP_003156.1).

Immunogen

Peptide with sequence C-SRVSFEDQAPTME, from the C Terminus of the protein sequence according to NP_003156.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70-75kDa band observed in Human Cerebellum lysates (calculated MW of 68.7kDa according to Human NP_003156.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

Specific Reference

This antibody has been successfully used in IHC on Mouse:
Meijer M, Cijsouw T, Toonen RF, Verhage M.
Synaptic Effects of Munc18-1 Alternative Splicing in Excitatory Hippocampal Neurons.
PLoS One. 2015 Sep 25;10(9):e0138950.
PMID: 26407320

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