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Goat Anti-NOGO-A / Reticulon 4 Antibody (EB09162)

Related Products: EBP09162 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09162 Goat Anti-NOGO-A / Reticulon 4 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09162 1
EB09162 (2.5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09162 2
EB09162 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RTN4, reticulon 4, ASY, NI220/250, NOGO, NOGO-A, NOGOC, NSP, NSP-CL, Nbla00271, Nbla10545, Nogo-B, Nogo-C, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, Human NogoA, My043 protein, foocen, neurite growth inhibitor 220, neurite outgrowth inhibitor, neuroendocrine-specific protein C homolog, reticulon 5
Official Symbol: RTN4
Accession Number(s): NP_065393.1; NP_997404.1
Human GeneID(s): 57142
Important Comments: This antibody is expected to recognize reported isoform A (NP_065393.1) and isoform E (NP_997404.1).

Immunogen

Peptide with sequence C-KAQIVTEKNTSTKTS, from the internal region of the protein sequence according to NP_065393.1; NP_997404.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
IHC: In paraffin embedded Human Cortex shows cytoplasm staining in neuronal cell bodies and in axons. Paraffin embedded Human Testis. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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