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Goat Anti-NOXO1 Antibody (EB06296)

Code Name Applications Availability Size Tested species Price Grade  
EB06296 Goat Anti-NOXO1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB06296 1
EB06296 (10µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NOXO1, P41NOX, P41NOXA, P41NOXB, P41NOXC, MGC20258, NADPH oxidase organizer 1, regulatory protein P41NOX, SH3PXD5, SNX28, Nox organizer 1
Official Symbol: NOXO1
Accession Number(s): NP_653204.1; NP_751907.1; NP_751907.1
Human GeneID(s): 124056
Important Comments: This antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).

Immunogen

Peptide with sequence RGCVDSVPHPTTEQ, from the C Terminus of the protein sequence according to NP_653204.1; NP_751907.1; NP_751907.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave no signal but low background in Hela and Human Heart lysates at up to 1µg/ml.
IHC: In paraffin embedded Human Kidney Cortex shows cytoplasmic stain in epithelial cells of some PCT. Recommended concentration, 10-20µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

PMID: 0

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