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Goat Anti-NPC2 Antibody (EB09947)

Related Products: EBP09947 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09947 Goat Anti-NPC2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09947 1
EB09947 (0.5µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: EDDM1, epididymal protein 1, epididymal secretory protein, epididymal secretory protein E1, HE1, MGC1333, Niemann-Pick disease, type C2, NP-C2, tissue-specific secretory protein, NPC2
Official Symbol: NPC2
Accession Number(s): NP_006423.1
Human GeneID(s): 10577

Immunogen

Peptide with sequence QAEPVQFKDCGSVD, from the internal region of the protein sequence according to NP_006423.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Experiments gave bands at approx 20kDa and 23kDa in Human Testis lysates. These bands correspond to earlier findings in literature with different antibodies (Légaré et al, Mol Hum Reprod. 2006 Jul;12(7):461-8; PMID: 16772431. This protein has a calculated MW of 16.6kDa according to NP_006423.1. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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