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Code Name Applications Availability Size Tested species Price Grade  
EB05067 Goat Anti-ORC4L Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05067 1
EB05067 (0.3µg/ml) staining of Jurkat nuclear lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ORC4L, origin recognition complex, subunit 4-like (yeast), Orc4p, HSORC4, origin recognition complex, subunit 4 (yeast homolog)-like, Origin recognition complex, subunit 4, S. cerevisiae, homolog-like, ORC4, ORC4P, origin recognition complex subunit 4, origin recognition complex, subunit 4 homolog
Official Symbol: ORC4
Accession Number(s): NP_002543.2; NP_001177810.1; NP_001177811.1
Human GeneID(s): 5000
Non-Human GeneID(s): 26428 (mouse), 295596 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_002543.2; NP_001177810.1; NP_001177811.1). Reported variants represent identical protein: NP_002543.2, NP_001177808.1, NP_859526.1, NP_859525.1

Immunogen

Peptide with sequence C-DVRQWATSSLSWL, from the C Terminus of the protein sequence according to NP_002543.2; NP_001177810.1; NP_001177811.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 48kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 50.4kDa according to Human NP_002543.2 Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on cell line NIH-3T3 and Mouse Heart for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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