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Goat Anti-Orexin Receptor 2 Antibody (EB08124)

Related Products: EBP08124 - Immunizing Peptide, EBP10833 - Immunizing Peptide, Goat Anti-Orexin Receptor 2 (aa405-419) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08124 Goat Anti-Orexin Receptor 2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08124 1
EB08124 (1.5µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: OTTHUMP00000039969, hypocretin (orexin) receptor 2, HCRTR2, OX2R, hypocretin receptor-2, orexin receptor 2
Official Symbol: HCRTR2
Accession Number(s): NP_001517.1
Human GeneID(s): 3062
Non-Human GeneID(s): 25605 (rat)

Immunogen

Peptide with sequence SGTKLEDSPPCRN, from the N Terminus of the protein sequence according to NP_001517.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 50kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 50.7kDa according to NP_001517.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in ICC on Mouse:
Danlei Liu et al.
Lateral hypothalamus orexinergic projection to the medial prefrontal cortex modulates chronic stress-induced anhedonia but not anxiety and despair.
Transl Psychiatry. 2024 Mar 16;14(1):149.
PMID: 38493173

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