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Goat Anti-P2X4 / P2X4R Antibody (EB08830)

Related Products: EBP08830 - Immunizing Peptide, EBP11413 - Immunizing Peptide, Goat Anti-P2rx4 (mouse) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08830 Goat Anti-P2X4 / P2X4R Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08830 1
EB08830 (1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08830 2
EB08830 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: P2X4, P2X4R, P2RX4, purinergic receptor P2X, ligand-gated ion channel, 4, ATP receptor, ATP-gated cation channel protein, P2X purinoceptor 4, P2X receptor, subunit 4, purinergic receptor P2X ligand-gated ion channel 4, purinergic receptor P2X4, purinoceptor P2X4
Official Symbol: P2RX4
Accession Number(s): NP_002551.2; NP_001243725.1; NP_001248326.1
Human GeneID(s): 5025
Non-Human GeneID(s): 18438 (mouse), 29659 (rat)

Immunogen

Peptide with sequence C-YREKKYKYVEDYEQ, from the C Terminus of the protein sequence according to NP_002551.2; NP_001243725.1; NP_001248326.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 60kDa band observed in Human Kidney lysates and in preliminary testing of Human Liver lysate (calculated MW of 43.4kDa according to NP_002551.2).This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific References

This antibody (previous batch) has been successfully used in the following paper:
Freeman KW, Bowman BR, Zetter BR.
Regenerative protein thymosin beta-4 is a novel regulator of purinergic signaling.
FASEB J. 2011 Mar;25(3):907-15.
PMID: 21106936

This antibody (previous batch) has been successfully used in the following paper:
Yuan H, Zhu X, Zhou S, Chen Q, Zhu X, Ma X, He X, Tian M, Shi X.
Role of mast cell activation in inducing microglial cells to release neurotrophin.
J Neurosci Res. 2010 May 1;88(6):1348-54.
PMID: 20025063

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