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Goat Anti-PACE4 Antibody (EB08962)

Code Name Applications Availability Size Tested species Price Grade  
EB08962 Goat Anti-PACE4 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

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EB08962 1
EB08962 (3.75µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08962 2
EB08962 (3.75µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PCSK6, proprotein convertase subtilisin/kexin type 6, PACE4, SPC4, paired basic amino acid cleaving system 4, subtilisin-like proprotein convertase 4, subtilisin-like protease, subtilisin/kexin-like protease PACE4
Official Symbol: PCSK6
Accession Number(s): NP_002561.1; NP_612192.1; NP_612195.1; NP_612197.1; NP_612196.1; NP_612198.2; NP_612193.1; NP_612194.1
Human GeneID(s): 5046
Non-Human GeneID(s): 18553 (mouse), 25507 (rat)
Important Comments: This antibody is expected to recognize reported isoforms a, b, g, h (NP_002561.1; NP_612192.1; NP_612193.1; NP_612194.1 resp.).

Immunogen

Peptide with sequence PDCEPGTYFDSE, from the internal region of the protein sequence according to NP_002561.1; NP_612192.1; NP_612195.1; NP_612197.1; NP_612196.1; NP_612198.2; NP_612193.1; NP_612194.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx. 70kDa band in Human, Mouse and Rat Liver lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 105 to 107kDa according to NP_002561.1, NP_612192.1, NP_612193.1 and NP_612194.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Testis and Skeletal Muscle. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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