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Goat Anti-PCDH11Y / PCDH11X Antibody (EB09719)

Related Products: EBP09719 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09719 Goat Anti-PCDH11Y / PCDH11X Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09719 1
EB09719 (6µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB09719 2
EB09719 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PCDH11Y, protocadherin 11 Y-linked, PCDH-PC, PCDH11X, PCDH22, PCDHY, OTTHUMP00000033077, protocadherin 11 X-linked, protocadherin 22, protocadherin Y, RP13-138P15.1, PCDH-X, PCDH11, PCDHX, protocadherin 11X, protocadherin X, protocadherin-S
Official Symbol: PCDH11Y
Accession Number(s): NP_116753.1; NP_116754.1; NP_116755.1; NP_055337.1; NP_055337.1; NP_116750.1; NP_116751.1
Human GeneID(s): 83259 , 27328
Important Comments: This antibody is expected to recognize all reported Y-linked isoforms (NP_116753.1; NP_116754.1; NP_116755.1) and all reported X-linked isoforms (NP_055337.1; NP_055337.1; NP_116750.1; NP_116751.1).

Immunogen

Peptide with sequence C-KYNWVTTPTTFK, from the internal region of the protein sequence according to NP_116753.1; NP_116754.1; NP_116755.1; NP_055337.1; NP_055337.1; NP_116750.1; NP_116751.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 75kDa band in Human Brain (Amygdala, Hippocampus, Substantia nigra) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115kDa according to NP_116753.1 and of 113kDa according to NP_055337.1, the smallest reported isoforms. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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