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Goat Anti-PDE5A Antibody (EB06645)

Related Products: EBP06645 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06645 Goat Anti-PDE5A Antibody Pep-ELISA, WB-Trf, IF, FC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

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EB06645 1
HEK293 overexpressing PDE5A (RC218974) and probed with EB06645 (mock transfection in first lane), tested by Origene.
EB06645 2
EB06645 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06645 3
EB06645 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Target Protein

Principal Names: PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3\1,5\1-cyclic nucleotide phosphodiesterase
Official Symbol: PDE5A
Accession Number(s): NP_001074.2; NP_246273.2; NP_236914.2; (NP_237223)
Human GeneID(s): 8654
Important Comments: This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.

Immunogen

Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2; NP_246273.2; NP_236914.2; (NP_237223).

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8kDa according to isoform 2, NP_236914.2). Recommended concentration: 0.3-1µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog, Cow