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Goat Anti-PDK1 Antibody (EB06983)

Related Products: EBP06983 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06983 Goat Anti-PDK1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06983 1
EB06983 (1µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PDK1, pyruvate dehydrogenase kinase, isoenzyme 1, HGNC:8809, mitochondrial pyruvate dehydrogenase kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1, OTTHUMP00000205076, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
Official Symbol: PDK1
Accession Number(s): NP_002601.1
Human GeneID(s): 5163

Immunogen

Peptide with sequence C-DFKDKSAEDAK, from the internal region of the protein sequence according to NP_002601.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 65-70kDa band observed in Rat Heart lysates (calculated MW of 49.2kDa according to NP_002601.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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