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Goat Anti-Pericentrin 1 / NUP85 Antibody (EB06201)

Code Name Applications Availability Size Tested species Price Grade  
EB06201 Goat Anti-Pericentrin 1 / NUP85 Antibody Pep-ELISA, WB, IP Yes 100µg specific antibody in 200µl Human $324.00 Elite

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EB06201 1
EB06201 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NUP85, pericentrin 1, PCNT1, PCN, PCNT, frount, nucleoporin Nup75, nucleoporin 85kDa, FLJ12549, Nup75, nucleoporin 85
Official Symbol: NUP85
Accession Number(s): NP_079120.1
Human GeneID(s): 79902
Non-Human GeneID(s): 18541 (mouse)
Important Comments: Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies).

Immunogen

Peptide with sequence C-ARAIIREGSLEGS, from the C Terminus of the protein sequence according to NP_079120.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Toda E et al, J Immunol. 2009 Nov 15;183(10):6387-94. PMID: 19841162.

Immunoprecipitation: This antibody was successfully used in IP on Human: Toda E et al, J Immunol. 2009 Nov 15;183(10):6387-94. PMID: 19841162.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Specific Reference

This antibody has been successfully used in WB and IP on Human:
Toda E, Terashima Y, Sato T, Hirose K, Kanegasaki S, Matsushima K.
FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration.
J Immunol. 2009 Nov 15;183(10):6387-94.
PMID: 19841162

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