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Code Name Applications Availability Size Tested species Price Grade  
EB07728 Goat Anti-Perilipin 1 (C Terminus) Antibody Pep-ELISA, WB Contact sales for delivery estimate 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB07728 1
EB07728 (2µg/ml) staining of Human Breast (A) and (1ug/ml) Breast cancer (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07728 2
EB07728 (1µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Plin, perilipin, PERI, lipid droplet-associated protein, PLIN1, Perilipin1, Perilipin 1
Official Symbol: PLIN1
Accession Number(s): NP_002657.3
Human GeneID(s): 5346
Non-Human GeneID(s): 25629 (rat)

Immunogen

Peptide with sequence C-EPILGRAQYSQLRKK, from the C Terminus of the protein sequence according to NP_002657.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55kDa band observed in Human Breast and Breast cancer lysates, and in lysates of cell line Jurkat (calculated MW of 56.0kDa according to NP_002657.3). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog

Specific References

This antibody (previous batch) has been successfully used in IHC on Mouse:
Rayanne B Burl, Elizabeth Ann Rondini, Hongguang Wei, Roger Pique-Regi, James G Granneman
Deconstructing cold-induced brown adipocyte neogenesis in mice.
Elife. 2022 Jul18;11:e80167.doi:10.7554/eLife.80167.
PMID: 35848799

This antibody (previous batch) has been successfully used in IF on Human:
Emilio P.Mottillo, Huamei Zhang, Alexander Yang, Li Zhou, James G.Granneman
Genetically-encoded sensors to detect fatty acid production and trafficking
Molecular Metabolism, Vol 29, Nov. 2019, https://doi.org/10.1016/j.molmet.2019.08.012
PMID: 31668392

This antibody (previous batch) has been successfully used in IHC and IF on Mouse:
Lee YH, Petkova AP, Konkar AA, Granneman JG.
Cellular origins of cold-induced brown adipocytes in adult mice.
FASEB J. 2015 Jan;29(1):286-99.
PMID: 25392270

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