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Goat Anti-PLCD3 (aa261-274) Antibody (EB11174)

Related Products: EBP11174 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11174 Goat Anti-PLCD3 (aa261-274) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11174 1
EB11174 (2µg/ml) staining of A549 (A) and Caco-2 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11174 2
EB11174 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB11174 3
EB11174 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
EB11174 4
EB11174 (6µg/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
EB11174 5
EB11174 Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3, MGC71172, phosphoinositide phospholipase C-delta-3, phospholipase C, delta 3, phospholipase C-delta-3, PLC delta3, PLC-delta-3, PLCD3
Official Symbol: PLCD3
Accession Number(s): NP_588614.1
Human GeneID(s): 113026
Non-Human GeneID(s): 72469 (mouse), 287745 (rat)

Immunogen

Peptide with sequence C-HQYSGEDRVLSAPE, from the internal region of the protein sequence according to NP_588614.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 85kDa band observed in lysates of cell limes A549 and Caco-2, and in preliminary testing of cell lines HeLa, HEK293 and U251 lysate (calculated MW of 89.3kDa according to NP_588614.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney and Testis. Recommended concentration: 6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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