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Goat Anti-PON2 Antibody (EB09835)

Related Products: EBP09835 - Immunizing Peptide, EBP09836 - Immunizing Peptide, Goat Anti-PON2 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09835 Goat Anti-PON2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09835 1
EB09835 (0.2µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09835 2
EB09835 (3.75µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: A-esterase 2, aromatic esterase 2, paraoxonase 2, paraoxonase nirs, serum aryldialkylphosphatase 2, serum paraoxonase/arylesterase 2, PON2
Official Symbol: PON2
Accession Number(s): NP_000296.2; NP_001018171.1
Human GeneID(s): 5445
Non-Human GeneID(s): 296851 (rat)
Important Comments: This antibody is expected to recognize isoforms 1 and 2 (NP_000296.2; NP_001018171.1).

Immunogen

Peptide with sequence C-EKPRARELRIS, from the internal region of the protein sequence according to NP_000296.2; NP_001018171.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa double band observed in lysates of HepG2 (calculated MW of 39.4kDa according to NP_000296.2 and of 38.0kDa according to NP_001018171.1). Recommended concentration: 0.2-0.6µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Pig, Dog

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