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Goat Anti-POU4F2 Antibody (EB10032)

Related Products: EBP10032 - Immunizing Peptide, EBP10033 - Immunizing Peptide, Goat Anti-POU4F2 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10032 Goat Anti-POU4F2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10032 1
EB10032 (1µg/ml) staining of Human Cerebellum (A) + peptide (B), Mouse Brain (C) + peptide (D), and Rat Brain (E) + peptide (F) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BRN3.2, BRN3B, Brn-3b, Brn3b POU domain transcription factor, POU class 4 homeobox 2, POU domain class 4 transcription factor 2, POU domain protein, POU domain, class 4, transcription factor 2, POU4F2
Official Symbol: POU4F2
Accession Number(s): NP_004566.2
Human GeneID(s): 5458
Important Comments: This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)

Immunogen

Peptide with sequence HGLPSHMGCMSDVD, from the internal region of the protein sequence according to NP_004566.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 35kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 43.1kDa according to Human NP_004566.2, ). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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