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Goat Anti-PRKACA Antibody (EB12865)

Code Name Applications Availability Size Tested species Price Grade  
EB12865 Goat Anti-PRKACA Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $324.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB12865 1
EB12865 (2µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PRKACA, protein kinase, cAMP-dependent, catalytic, alpha, PKACA, PPNAD4, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha, protein kinase A catalytic subunit
Official Symbol: PRKACA
Accession Number(s): NP_002721.1; NP_997401.1; NP_001291278.1
Human GeneID(s): 5566
Important Comments: This antibody is expected to recognize all three reported isoforms (NP_002721.1; NP_997401.1; NP_001291278.1). Based on the choice of the immunizing peptide there is no expected cross-reactivity with the other subunits.

Immunogen

Peptide with sequence C-KHKETGNHYAMK, from the internal region of the protein sequence according to NP_002721.1; NP_997401.1; NP_001291278.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 40kDa band observed in lysates of cell line HeLa (calculated MW of 40.6kDa according to NP_997401.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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