Change country
Home
Code Name Applications Availability Size Tested species Price Grade  
EB11136 Goat Anti-PRODH (aa112-134) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11136 1
EB11136 (0.3µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: FLJ33744, HSPOX2, MGC148078, MGC148079, OTTHUMP00000196496, OTTHUMP00000196497, p53-induced gene 6 protein, PIG6, POX, PRODH1, PRODH2, proline dehydrogenase (oxidase) 1, proline dehydrogenase, mitochondrial, proline oxidase 2, proline oxidase, mitochondrial, SCZD4, TP53I6, tumor protein p53 inducible protein 6, PRODH
Official Symbol: PRODH
Accession Number(s): NP_057419.4; NP_001182155.1
Human GeneID(s): 5625
Important Comments: This antibody is expected to recognize both reported isoforms (NP_057419.4; NP_001182155.1).

Immunogen

Peptide with sequence C-EDQESIQPLLRHYR, from the internal region of the protein sequence according to NP_057419.4; NP_001182155.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx75+55kDa bands observed in Human Brain (Cerebellum and Substantia nigra) lysates (calculated MW of 68.0kDakDa according to NP_057419.4 and of 56.2kDa according to NP_001182155.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific References

This antibody has been successfully used in WB on Human:
Ilona Oscilowska, Thi Y. L. Huynh, Weronika Baszanowska, Izabela Prokop, Arkadiusz Surazynski, Mauro Galli, Piotr Zabielski & Jerzy Palka
Proline oxidase silencing inhibits p53-dependent apoptosis in MCF-7 breast cancer cells.
Amino Acids. 2021 Dec;53(12):1943-1956.
PMID: 34085157

This antibody has been successfully used in Western blot on Human:
Zareba I, Surazynski A, Chrusciel M, Miltyk W, Doroszko M, Rahman N, Palka J.
Functional Consequences of Intracellular Proline Levels Manipulation Affecting PRODH/POX-Dependent Pro-Apoptotic Pathways in a Novel in Vitro Cell Culture Model.
Cell Physiol Biochem. 2017 Sep 22;43(2):670-684.
PMID: 28942439

Product Reviews

Please login to review this product.