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Code Name Applications Availability Size Tested species Price Grade  
EB07725 Goat Anti-PTPN6 / SHP1 (internal region) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07725 1
EB07725 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07725 2
EB07725 (0.01µg/ml) staining of Mouse Thymus (A) and (0.1ug/ml) Rat Thymus (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07725 3
EB07725 (5µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTPN6, SHP1, protein tyrosine phosphatase, non-receptor type 6, HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, 70 kda SHP-1L protein, hematopoietic cell phosphatase, hematopoietic cell protein-tyrosine phosphatase, protein-tyrosine phosphatase 1C
Official Symbol: PTPN6
Accession Number(s): NP_536858.1; NP_002822.2
Human GeneID(s): 5777
Non-Human GeneID(s): 15170 (mouse), 116689 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_536858.1and NP_002822.2). Please note this antibody was designed using the mouse sequence, which differs by 1 amino acid from the human sequence.

Immunogen

Peptide with sequence C-KASRTSSKHKEE, from the internal region of the protein sequence according to NP_536858.1; NP_002822.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 70kDa band observed in Human Liver and Mouse Thymus lysates and approx 70-75kDa in Rat Thymus lysates (calculated MW of 67.6kDa according to Human NP_536858.1 and Mouse NP_038573.2, and 69.6kDa according to Rat NP_446360.1). Recommended concentration: 0.01-0.1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Thyroid. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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