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Goat Anti-RAD51C Antibody (EB05686)

Related Products: EBP05686 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05686 Goat Anti-RAD51C Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05686 1
EB05686 staining (2µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: RAD51C, RAD51 homolog C (S. cerevisiae), RAD51L2, yeast RAD51 homolog 3, DNA repair protein RAD51 homolog 3, MGC104277, RAD51 homolog C, RAD51 homolog C, isoform 1
Official Symbol: RAD51C
Accession Number(s): NP_002867.1; NP_478123.1
Human GeneID(s): 5889
Important Comments: This antibody is expected to recognise all reported human isoforms.

Immunogen

Peptide with sequence RGKTFRFEMQRDL-C, from the N Terminus of the protein sequence according to NP_002867.1; NP_478123.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 45kDa band seen in HeLa lysate [Predicted MW of 41kDa according to NP_478123]. Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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