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Goat Anti-RASAL Antibody (EB06176)

Code Name Applications Availability Size Tested species Price Grade  
EB06176 Goat Anti-RASAL Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB06176 1
EB06176 (0.3µg/ml) staining of Human Thyroid lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Target Protein

Principal Names: RASAL1, RASAL, RAS protein activator like 1 (GAP1 like), GAP1 like protein, RAS-GTPase-activating protein-like, RAS protein activator like 1
Official Symbol: RASAL1
Accession Number(s): NP_004649.1
Human GeneID(s): 8437

Immunogen

Peptide with sequence C-QERGKAALGPLGP, from the C Terminus of the protein sequence according to NP_004649.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90kDa band observed in Human Thyroid gland lysates (calculated MW of 90.0kDa according to NP_004649.1). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 21kDa. This band was successfully blocked by incubation with the immunising peptide.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human