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Goat Anti-RIG1 / RARRES3 (aa 124 to 136) Antibody (EB08339)

Related Products: EBP07056 - Immunizing Peptide, EBP08339 - Immunizing Peptide, Goat Anti-RIG1 / RARRES3 (aa 119 to 134) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08339 Goat Anti-RIG1 / RARRES3 (aa 124 to 136) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08339 1
EB08339 (3.75µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RARRES3, retinoic acid receptor responder (tazarotene induced) 3, HRASLS4, MGC8906, RIG1, TIG3
Official Symbol: PLAAT4
Accession Number(s): NP_004576.2
Human GeneID(s): 5920

Immunogen

Peptide with sequence KSRCKQVEKAKVE, from the internal region of the protein sequence according to NP_004576.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave bands at approx 22kDa and 55kDa in Human Thymus lysates after 1µg/ml antibody staining. The 55kDa band was observed in all tissues tested. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 18.8kDa according to NP_004576.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: Paraffin embedded Human Placenta. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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