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Goat Anti-MST4 Antibody (EB05368)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05368 | Goat Anti-MST4 Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB05368 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05368 (2.5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Target Protein
Principal Names: RP6-213H19.1, serine/threonine protein kinase MST4, MASK, MST4, Mst3 and SOK1-related kinase, STE20-like kinase MST4, mammalian Ste20-like protein kinase 4, mammalian sterile 20-like 4, serine/threonine protein kinase MASKOfficial Symbol: STK26
Accession Number(s): NP_057626.2; NP_001035918.1; NP_001035917.1
Important Comments: This antibody is expected to recognize all three reported isoforms (NP_057626.2, NP_001035918.1 and NP_001035917.1).
Immunogen
Peptide with sequence KLIEKFQKCSADESP, from the C Terminus of the protein sequence according to NP_057626.2; NP_001035918.1; NP_001035917.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 50kDa band observed in lysates of Jurkat cell lines (calculated MW of 46.5kDa according to NP_057626.2). Recommended concentration: 0.5-1.5µg/ml.
IHC: Paraffin embedded Human Thymus. Recommended concentration: 2.5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow