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Goat Anti-SART3 Antibody (EB05744)

Related Products: EBP05744 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05744 Goat Anti-SART3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB05744 1
EB05744 staining (0.01µg/ml) of Jurkat cell lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SART3, squamous cell carcinoma antigen recognised by T cells 3, TIP110, KIAA0156, p110(nrb), RP11-13G14, tat-interacting protein of 110 kDa, DSAP1, MGC138188, P100, p110
Official Symbol: SART3
Accession Number(s): NP_055521.1
Human GeneID(s): 9733

Immunogen

Peptide with sequence C-MSNADFAKLFLRK, from the C Terminus of the protein sequence according to NP_055521.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 170kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 111kDa according to NP_055521.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yang et al, Cancer Res. 1999 Aug 15;59(16):4056-63; PMID: 10463607). Recommended concentration: 0.01-0.03µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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