Change country
Home
Code Name Applications Availability Size Tested species Price Grade  
EB08932 Goat Anti-SEPT6 Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08932 1

EB08932 optimised QC. Primary incubation 1 hour at room temperature.

Images A, B, C: Daudi, Jurkat, MOLT-4 cell lysates at primary Ab concentration 0.1ug/ml (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB08932 2

EB08932 optimised QC. Primary incubation 1 hour at room temperature.

Image A: Human Testes lysate at primary Ab concentration 1ug/ml, Image B: Human Duodenum lysate at primary Ab concentration 0.1ug/ml, Image C: Human Kidney lysate at primary Ab concentration 0.5ug/ml, Image D: Human Tonsil lysate at primary Ab concentration 0.3ug/ml (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB08932 3
EB08932 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08932 4
EB08932 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08932 5
EB08932 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB08932 6
EB08932 (3.8µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SEPT6, septin 6, KIAA0128, MGC16619, MGC20339, SEP2, SEPT2, septin 2
Official Symbol: SEPTIN6
Accession Number(s): NP_665798.1; NP_055944.2; NP_665801.1
Human GeneID(s): 23157
Important Comments: This antibody is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein.

Immunogen

Peptide with sequence C-DEVNAFKQRKTA, from the internal region of the protein sequence according to NP_665798.1; NP_055944.2; NP_665801.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 50kDa band observed in Human Testis, Duodenum, Kidney and Tonsil lysates, and in lysates of cell lines Daudi, Jurkat and MOLT-4 (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration: 3-5µg/ml.

Immunofluorescence: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

Product Reviews

Please login to review this product.