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Goat Anti-Serotonin Receptor 3B / HTR3B Antibody (EB09495)

Code Name Applications Availability Size Tested species Price Grade  
EB09495 Goat Anti-Serotonin Receptor 3B / HTR3B Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB09495 1
EB09495 (1µg/ml) staining of Human Cerebral Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HTR3B, 5-hydroxytryptamine (serotonin) receptor 3B, 5-HT3B, 5-hydroxytryptamine 3 receptor B subunit, serotonin-gated ion channel subunit
Official Symbol: HTR3B
Accession Number(s): NP_006019.1
Human GeneID(s): 9177

Immunogen

Peptide with sequence C-SNYLQTQDQTDQQE, from the internal region (near C-terminus) of the protein sequence according to NP_006019.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 51kDa band observed in Human Brain (Cerebral Cortex, Hippocampus and Amygdala) lysates (calculated MW of 50.3kDa according to NP_006019.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human