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Goat Anti-SLC12A1 / NKCC Antibody (EB09143)

Related Products: EBP09143 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09143 Goat Anti-SLC12A1 / NKCC Antibody Pep-ELISA, WB, IHC, IF, ICC Yes 100µg specific antibody in 200µl Human, Mouse, Pig $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB09143 1
EB09143 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SLC12A1, solute carrier family 12 (sodium/potassium/chloride transporters), member 1, BSC1, MGC48843, NKCC2, NKCC2A variant A, Na-K-2Cl cotransporter, sodium potassium chloride cotransporter 2
Official Symbol: SLC12A1
Accession Number(s): NP_000329.2
Human GeneID(s): 6557

Immunogen

Peptide with sequence C-PHYEETSFGDEAQKR, from the internal region of the protein sequence according to NP_000329.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: This anitbody has been successfully used in WB on Mouse: Alshahrani S et al. (2015) PMID: 26400961.
IHC: In paraffin embedded Human Kidney shows strong membrane staining in some CT cells. Recommended concentration, 3-6µg/ml.

IF/ICC: This antibody has been successfully used in IF on Mouse: Alshahrani S et al. (2015) PMID: 26400961. This antibody has been successfully used in ICC on Pig: Andreas Brännström et al. (2022) PMID: 36471055.

Species Reactivity

Tested: Human, Mouse, Pig
Expected from sequence similarity: Human

Specific References

This antibody has been successfully used in ICC on Pig:
Andreas Brännström, Michael Hultström, Jenny Gustavsson, Zabih Aurfan & Mattias Günther
Intermittent thoracic resuscitative endovascular balloon occlusion of the aorta improves renal function compared to 60 min continuous application after porcine class III hemorrhage.
Eur J Trauma Emerg Surg (2022). https://doi.org/10.1007/s00068-022-02189-2
PMID: 36471055

This antibody has been successfully used in WB and IF in Mouse:
Alshahrani S, Almutairi MM, Kursan S, Dias-Junior E, Almiahuob MM, Aguilar-Bryan L, Di Fulvio M.
Increased Slc12a1 expression in β-cells and improved glucose disposal in Slc12a2 heterozygous mice.
J Endocrinol. 2015 Dec;227(3):153-65.
PMID: 26400961

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