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Goat Anti-SMN1 / SMN2 Antibody (EB07704)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07704 | Goat Anti-SMN1 / SMN2 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB07704 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Target Protein
Principal Names: SMN1, survival of motor neuron 1, telomeric, BCD541, SMA1, SMA2, SMA3, SMA4, SMN, SMNT, T-BCD541, gemin 1, SMN2, survival of motor neuron 2, centromeric, BCD541, C-BCD541, SMNCOfficial Symbol: SMN1 / SMN2
Accession Number(s): NP_075012.1; NP_000335.1; NP_075013.1; NP_075014.1; NP_075015.1; NP_059107.1
Human GeneID(s): 6606 , 6607
Important Comments: This antibody is expected to recognise isoforms b and d of SMN1 (NP_075012.1 and NP_000335.1) and all reported isoforms of SMN2 (NP_075013.1, NP_075014.1, NP_075015.1 and NP_059107.1).
Immunogen
Peptide with sequence C-DESENSRSPGNKSDN, from the internal region of the protein sequence according to NP_075012.1; NP_000335.1; NP_075013.1; NP_075014.1; NP_075015.1; NP_059107.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 26kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 28.5kDa according to NP_075012.1 and NP_075014.1). Recommended concentration: 0.3-1µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human