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Goat Anti-SMN1 / SMN2 Antibody (EB07704)

Related Products: EBP07704 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07704 Goat Anti-SMN1 / SMN2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07704 1
EB07704 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SMN1, survival of motor neuron 1, telomeric, BCD541, SMA1, SMA2, SMA3, SMA4, SMN, SMNT, T-BCD541, gemin 1, SMN2, survival of motor neuron 2, centromeric, BCD541, C-BCD541, SMNC
Official Symbol: SMN1 / SMN2
Accession Number(s): NP_075012.1; NP_000335.1; NP_075013.1; NP_075014.1; NP_075015.1; NP_059107.1
Human GeneID(s): 6606 , 6607
Important Comments: This antibody is expected to recognise isoforms b and d of SMN1 (NP_075012.1 and NP_000335.1) and all reported isoforms of SMN2 (NP_075013.1, NP_075014.1, NP_075015.1 and NP_059107.1).

Immunogen

Peptide with sequence C-DESENSRSPGNKSDN, from the internal region of the protein sequence according to NP_075012.1; NP_000335.1; NP_075013.1; NP_075014.1; NP_075015.1; NP_059107.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 28.5kDa according to NP_075012.1 and NP_075014.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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