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Goat Anti-SORL1 / LR11 (C Terminus) Antibody (EB05045)

Related Products: EBP05045 - Immunizing Peptide, EBP07713 - Immunizing Peptide, Goat Anti-SORL1 / LR11 (internal) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05045 Goat Anti-SORL1 / LR11 (C Terminus) Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05045 1
EB05045 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic with endosome staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05045 2
EB05045 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/endosome, membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: SORL1, LR11, sortilin-related receptor, L(DLR class) A repeats-containing, LRP9, SORLA, gp250, SorLA-1, mosaic protein LR11, C11orf32, FLJ21930, FLJ39258, sortilin-related receptor containing LDLR class A repeats
Official Symbol: SORL1
Accession Number(s): NP_003096.1
Human GeneID(s): 6653

Immunogen

Peptide with sequence C-TGFSDDVPMVIA, from the C Terminus of the protein sequence according to NP_003096.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.

Immunofluorescence: Strong expression of the protein seen in A431 and HepG2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in Mouse in the following paper:
Kim E, Lee Y, Lee HJ, Kim JS, Song BS, Huh JW, Lee SR, Kim SU, Kim SH, Hong Y, Shim I, Chang KT.
Implication of mouse Vps26b-Vps29-Vps35 retromer complex in sortilin trafficking.
Biochem Biophys Res Commun. 2010 Dec 10;403(2):167-71.
PMID: 21040701

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