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Goat Anti-SPON2 (301-315) Antibody (EB11284)

Related Products: EBP09965 - Immunizing Peptide, EBP11284 - Immunizing Peptide, Goat Anti-Spondin 2 / Mindin Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11284 Goat Anti-SPON2 (301-315) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11284 1
EB11284 (0.5µg/ml) staining of Human (A), Mouse (B) and Rat (C) Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11284 2
EB11284 (5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: differentially expressed in cancerous and non-cancerous lung cells 1, DIL1, DIL-1, DKFZp686G21139, FLJ16313, FLJ22401, FLJ34460, MINDIN, M-SPONDIN, spondin 2, extracellular matrix protein, spondin-2, SPON2
Official Symbol: SPON2
Accession Number(s): NP_036577.1
Human GeneID(s): 10417
Non-Human GeneID(s): 100689 (mouse), 171569 (rat)
Important Comments: Reported variants represent identical protein: NP_036577.1, NP_001121797.1, NP_001185950.1

Immunogen

Peptide with sequence C-RTRYVRVQPANNGSP, from the internal region (near C Terminus) of the protein sequence according to NP_036577.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 35-36kDa band observed in Human, Mouse and Rat Kidney lysates, and in preliminary testing of A549 cell lysates (calculated MW of 35.8kDa according to Human NP_036577.2,and 36.0kDa according to Mouse NP_598664.3 and Rat NP_612542.1). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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