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Goat Anti-Stomatin Antibody (EB11981)

Code Name Applications Availability Size Tested species Price Grade  
EB11981 Goat Anti-Stomatin Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11981 1
EB11981 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB11981 2
EB11981 (0.1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: STOM, stomatin, BND7, EPB7, EPB72, erythrocyte band 7 integral membrane protein, erythrocyte membrane protein band 7.2 (stomatin), protein 7.2b
Official Symbol: STOM
Accession Number(s): NP_004090.4; NP_937837.1
Human GeneID(s): 2040
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004090.4; NP_937837.1).

Immunogen

Peptide with sequence EKRHTRDSEAQR-C, from the N Terminus of the protein sequence according to NP_004090.4; NP_937837.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 30kDa band observed in Human Erythrocytes, Spleen, Bone Marrow and Lung lysates (calculated MW of 31.7kDa according to NP_004090.4). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Liver shows membrane staining of hepatocytes and of sinusoidal lining cells. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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