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Code Name Applications Availability Size Tested species Price Grade  
EB10884 Goat Anti-STS2 / TULA (aa470-482) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10884 1
EB10884 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10884 2
EB10884 (3.8µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBASH3A, ubiquitin associated and SH3 domain containing A, CLIP4, STS-2, TULA, OTTHUMP00000109368, T-cell ubiquitin ligand protein, cbl-interacting protein 4, suppressor of T-cell receptor signaling 2
Official Symbol: UBASH3A
Accession Number(s): NP_061834.1; NP_001001895.1
Human GeneID(s): 53347
Important Comments: This antibody is expected to recognize both reported isoforms (NP_061834.1; NP_001001895.1).

Immunogen

Peptide with sequence C-DSGIRISSVFASP, from the internal region of the protein sequence according to NP_061834.1; NP_001001895.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 70kDa band observed in Human Spleen lysates (calculated MW of 69.8kDa according to NP_001001895.1). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Breast shows perinuclear staining in the epithelial cells of the lubules. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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