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Code Name Applications Availability Size Tested species Price Grade  
EB07827 Goat Anti-TGM2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07827 1
EB07827 (0.1µg/ml) staining of K562 (A) and A549 (B) and (0.3ug/ml) Caco-2 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07827 2
EB07827 (0.1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07827 3
EB07827 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TGM2, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase), TG2, TGC, C polypeptide, TGase C, TGase-H, protein-glutamine-gamma-glutamyltransferase, tissue transglutaminase, transglutaminase 2, transglutaminase C
Official Symbol: TGM2
Accession Number(s): NP_004604.2; NP_001310245.1; NP_001310247.1; NP_001310246.1
Human GeneID(s): 7052
Important Comments: This antibody is expected to recognise iall reported isoforms.

Immunogen

Peptide with sequence C-NFESDKLKAVKGFR, from the internal region (near the C Terminus) of the protein sequence according to NP_004604.2; NP_001310245.1; NP_001310247.1; NP_001310246.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 70kDa band observed in K562, 70+80kDa in Caco-2 and 70+85kDa in A549 cell lysates, and approx. 80kDa in Human Placenta lysates (calculated MW of 70.3kDa according to NP_001310247.1 and 77.3kDa according to NP_004604.2). All observed molecular weights are reported by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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