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Code Name Applications Availability Size Tested species Price Grade  
EB07852 Goat Anti-TGM2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07852 1
EB07852 (0.1µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminecence.
EB07852 2
EB07852 (0.03µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Detected by chemiluminecence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TGM2, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase), TG2, TGC, C polypeptide, TGase C, TGase-H, protein-glutamine-gamma-glutamyltransferase, tissue transglutaminase, transglutaminase 2, transglutaminase C
Official Symbol: TGM2
Accession Number(s): NP_004604.2; NP_001310245.1; NP_001310247.1; NP_001310246.1
Human GeneID(s): 7052
Important Comments: This antibody is expected to recognise all reported isofoms.

Immunogen

Peptide with sequence C-ESDKLKAVKGFRN, from the C Terminus of the protein sequence according to NP_004604.2; NP_001310245.1; NP_001310247.1; NP_001310246.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 85kDa band observed in Human Placenta lysates, and approx. 80kDa in lysates of cell line A549 and in preliminary testing of Human pancreatic cancer lysate (calculated MW. of 77.3kDa according to NP_004604.2). These molecular weights are observed by other commercial sources. Recommended concentration: 0.01-0.1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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