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Goat Anti-TPD52L2 / D54 Antibody (EB08025)

Related Products: EBP08025 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08025 Goat Anti-TPD52L2 / D54 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB08025 1
EB08025 (0.1µg/ml) staining of Human Breast Cancer lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08025 2
EB08025 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08025 3
EB08025 (3.75µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08025 4
EB08025 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TPD52L2, D54, tumor protein D52-like 2, DKFZp686A1765, hD54, HCCR-binding protein 2
Official Symbol: TPD52L2
Accession Number(s): NP_955392.1; NP_955393.1; NP_955394.1; NP_955395.1; NP_003279.2; NP_955391.1
Human GeneID(s): 7165
Important Comments: This antibody is expected to recognise all reported isoforms (NP_955392.1; NP_955393.1; NP_955394.1; NP_955395.1; NP_003279.2; NP_955391.1).

Immunogen

Peptide with sequence C-SGDKPLSDPAP, from the C Terminus of the protein sequence according to NP_955392.1; NP_955393.1; NP_955394.1; NP_955395.1; NP_003279.2; NP_955391.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 28kDa band observed in Human Breast Cancer and Human Brain lysates (calculated MW of 24.9kDa according to NP_955392.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. See also our D52 and D53 products.
IHC: Paraffin embedded Human Spleen, Tonsil and Brain (Cortex). Recommended concentration: 3.75µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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